Rapid, reproducible enzyme immunoassay for gentamicin.
نویسندگان
چکیده
An enzyme immunoassay for gentamicin utilizing glucose-6-phosphate dehydrogenase was compared to the radioimmunoassay. The enzyme immunoassay for gentamicin was accurate, specific, and easily performed. It offers an ulternative method to assay aminoglycosides and could be used in institutions using enzyme immunoassay to assay other drugs.
منابع مشابه
Assay of netilmicin, using enzyme immunoassay for gentamicin.
A homogenous enzyme immunoassay for the quantitative determination of netilmicin in serum was developed. The procedure utilizes a commercially available assay for gentamicin (EMIT; Syva Co., Palo Alto, Calif.). The method was adapted to a microcentrifugal analyzer, and log-logit regression analysis was performed with a computer. The results of samples assayed by this method correlate well with ...
متن کاملEvaluation of five gentamicin assay procedures for clinical microbiology laboratories.
Five gentamicin assay procedures (a bioassay, an enzyme immunoassay, a latex agglutination inhibition test, a fluorescence immunoassay, and a radioimmunoassay) were evaluated to determine which was optimal for our laboratory. The evaluation was based on recovery and precision studies and results of analyses of patient samples, as well as technical assay performance factors. The latex agglutinat...
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The microbiological bioassay, the adenylation method, and the radiometric, enzyme, and fluorescent immunoassay methods for assaying serum gentamicin were compared. The precision, reproducibility, and specificity of each method was assessed and proved satisfactory, with the exception of the radioimmunoassay, which gave artificially high results. Good correlations between the other four methods w...
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An enzyme immunoassay for tobramycin utilizing glucose 6-phosphate dehydrogenase was compared with the radioimmunoassay. The enzyme immunoassay for tobramycin was accurate, specific, and easily performed. It offers an alternative method for assaying aminoglycosides and could be used in institutions that use the enzyme immunoassay to assay other drugs.
متن کاملPotential liabilities of gentamicin homogeneous enzyme immunoassay.
We report two potential liabilities of the homogeneous enzyme immunoassay for the determination of serum gentamicin. One is a considerable loss of precision and accuracy at the ends of the calibration curve, and the other is an apparent loss of gentamicin with storage at -60 degrees C.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 11 3 شماره
صفحات -
تاریخ انتشار 1980